Question & Answer: You have a protein solution of concentration 1 M. You need to make a series of six dilutions of…..

I need help with these. Please try to show as much work possible! Thank you.

You have a protein solution of concentration 1 M. You need to make a series of six dilutions of 200 mM, 40 mM, 10 mM, 200 mu M, 40 mu M, and 10 mu M. Starting with the highest concentration, use the previously dilution to make the next dilution, and so on. You will need at least 6 mL of each concentration to conduct the experiment, so be sure to make extra. A harder one which requires a little more algebra. You have 500.0 mL of dH2O. How much glycerine would you have to add in order to make a 5.0% (v/v) dilution?

7)

Don't use plagiarized sources. Get Your Custom Essay on
Question & Answer: You have a protein solution of concentration 1 M. You need to make a series of six dilutions of…..
GET AN ESSAY WRITTEN FOR YOU FROM AS LOW AS \$13/PAGE

1M = 1000mM

Take 6 ml of 1000mM and dilute it to 200mM

We can calculate the volume of 200mM protein by using formula

V1N1=V2N2

6ml x 1000nM = V2 x 200mM

V2 = 6ml x 1000nM / 200mM = 30 ml

Now dilute 30 ml 200mM protein to 40mM

30 ml x 200mM = V3 x 40 mM

V3 = 30 ml x 200mM / 40 mM = 150 ml

Dilute 150 ml 40mM protein solution to 10 mM

150 ml x 40 mM = V4 x 10 mM

V4 = 150 ml x 40mM / 10 mM = 600 ml

Dilute 600 ml 10mM protein solution to 2000 µM

1mM = 1000 µM

10mM = 10000 µM

600 ml x 10000 mM = V5 x 200 µM

V5 = 600 ml x 10000 mM /200 µM = 30000 ml

Dilute 3000 ml 200 µM protein solution to 40 µM

3000 ml x 200 µM = V6 x 40 µM = 15000 ml

Dilute 15000 ml 40 µM protein solution to 10 µM

15000 ml x 40 µM = V7 x 10 µM

V7 = 15000ml x 40 µM /10 µM = 60000 ml

After 6 series of dilution volume of protein solution increased 10000 times.

6 ml 1M protein solution gives 6.0L (60000ml) of 10 µM protein solution