 # Question & Answer: The first step in the procedure asks you to prepare five dilutions of the protein standard (BSA)…..

The first step in the procedure asks you to prepare five dilutions of the protein standard (BSA) in the range of 0.2 to 1.0 mg/ml. For each concentration, a volume of 1.0 mL should be prepared. Describe how you will prepare these standards using a stock solution of BSA that is 2.0 mg/ml.

A/C to M1V1 = M2V2

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V1 = M2V2/M1

M1 = 2 mg/mL , V2 = 1 mL,

Five dilutions in the range 0.2 to 1means that each dilution differ by 0.2 and concentrations are 0.2, 0.4, 0.6, 0.8 and 1 mg /mL.

For 0.2 mg/mL:

V1 = 0.2 x 1/ 2 = 0.1 mL

For 0.4 mg /mL:

V1 = 0.4 x 1/ 2 = 0.2 mL

For 0.6 mg /mL

V1 = 0.6 x 1/2 = 0.3 mL

For 0.8 mg/mL

V1 = 0.8 x 1/ 2 = 0.4 mg/mL

For 1mg /mL

V1 = 1×1/ 2 = 0.5 mg /mL

To prepare 0.2, 0.4, 0.6, 0 8 and 1 mg/mL of solutions we need to dissolve 0.1,0.2 0.3, 0.4 and 0.5 mL of stock solution in 1 mL of solvent (water) respectively.