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Suppose you are studying the activity of the enzyme that requires you to carry out the reaction in a solution that contains 20 mM Tris (pH 7.5), 100 mM NaCl and 0.05% (w/v) glycerol. Describe iin detail how you will prepare 100 mL of this buffer using the 0.5M Tris, solid sodium chloride, and liquid glycerol.
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